in Debugging styles in ext. css not on screen, or browser only des

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I have a web project in NET2.0/VS 2005 C#

this question is not about CSS instead why using asp.net 2.0 csharp when I
am debugging, the styles defined in the Style Sheets are not appearing on the
screen. They show in the designer, but when I debug (OR look at it in the
browser), they're gone. Doesn't change.

in css stylesheet one style

LABEL
{
font-size: 10pt;
color: red;
font-family: Verdana;
}
The stylesheet is in the App_Themes folder.
When I drag the css onto the aspx I get
<link href="App_Themes/StyleSheet.css" rel="stylesheet" type="text/css" />
this allows the styles to be seen but only in design mode

changing to
<link type="text/css" href="../App_Themes/StyleSheet.css" rel="stylesheet" />
css not seen at all

changing to
<link type="text/css" href="~/App_Themes/StyleSheet.css" rel="stylesheet" />
css not seen at all

of course inline style is seen, no other css is in the project
css is turning to a nightmare
Any help is appreciated, because I'm stumped!

cindy
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cindy