Web Service not being called

[Maraj]

Hello eveyone.
I have a problem calling webservice.I have made a webservice and added it in IIS as an application in a website.And i have to call it in same project hosted in production and problem is when i call it,it does not work.When i call it from my local PC it works.i.e My domain in which i have added webservice as an application is like Mydomain.com/MyService/service.asmx When i run it from my local PC it is being called but when i call it from production enviorment it does not work.I have plcaed the folder App_Webreferences generated from local PC into that website.What could be the probelem i get this error "Aconnection attempt failed because the connected party did not properly respond after a period of time, or established connection failed because connected host has failed to respond ".Any ideas please help.
Here is my web.config

<defaultProxy> 
        <!--<proxy autoDetect="True"/>--> 
        <proxy usesystemdefault="True" proxyaddress="Mydomain.com/MyService/service.asmx" bypassonlocal="True" autoDetect="True"/> 
    </defaultProxy> 
<appSettings> 
<add key="Email_Web.WebService value="http://Mydomain.com/MyService/service.asmx"/>  
</appSettings>